The LBC Reaction (leuco-brilliant cresyl blue reaction), a New Method to Demonstrate Mitochondria in Living Cells
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چکیده
منابع مشابه
Brilliant Cresyl Blue in Sap of Nitella
It has been shown by several investigators I that when the pl-I value of the external dye solutions is increased, the rate of accumulation of a basic dye in the cell sap is accelerated. Two different explanations for this have been given. Some have accounted for it on the basis that the basic dye enters the cell in the form of a dye hydrate and combines with the substances in the cell; and that...
متن کاملAccumulation of Brilliant Cresyl Blue in the Sap of Living Cells of Nitella in the Presence of Nh3
When the living cells of Nitella are placed in a solution of brilliant cresyl blue containing NH(4)Cl, the rate of accumulation of the dye in the sap is found to be lower than when the cells are placed in a solution of dye containing no NH(4)Cl and this may occur without any increase in the pH value of the cell sap. This decrease is found to be primarily due to the presence of NH(3) in the sap ...
متن کاملSelection of Ovine Oocytes by Brilliant Cresyl Blue Staining
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers...
متن کاملSPECTROPHOTOMETRIC REACTION RATE METHOD FOR THE DETERMINATION OF NITRITE BY CATALYTIC ACTION ON NILE BLUE A BROMATE REACTION
A kinetic method developed for the determination of ultra-trace amounts of nitrite based on its catalytic effect on the reaction between potassium bromate and Nile blue A in strongly acidic media is reported. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of Nile blue A at 645 nm by the fixed-time method. The decrease in absorbance during the first...
متن کاملOn-spot brilliant cresyl blue staining in fine needle aspiration, a novel technique for cytological diagnosis.
OBJECTIVE Application of on-spot staining with brilliant cresyl blue, a supravital stain, was investigated in fine needle aspirates from breast, lymph node and thyroid. STUDY DESIGN Patients with palpable breast lumps, lymph nodes and thyroid nodules were included in the study. Brilliant cresyl blue was used to stain the smears, which were evaluated for smear adequacy as well as cytological d...
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ژورنال
عنوان ژورنال: CYTOLOGIA
سال: 1964
ISSN: 0011-4545,1348-7019
DOI: 10.1508/cytologia.29.424